- Angelique Lamaze, Chenghao Chen, Solene Leleux, Min Xu, Rebekah George, Ralf Stanewsky. A natural timeless polymorphism allowing circadian clock synchronization in “white nights”. Nature Communications, 2022; 13 (1) DOI: 10.1038/s41467-022-29293-6
The study, which has now been published in the journal Nature Communications, focused on a clock gene called “timeless.” Together with a second clock gene (“period”), it controls the circadian rhythm of the fruit fly, which lasts about 24 hours. This rhythm is permanently synchronised with the environment using external zeitgebers such as light and temperature. The researchers investigated why a special variant of the timeless clock gene, namely the ls-tim allele, has spread so rapidly.
“Like humans, Drosophila originated in sub-Saharan Africa and spread North, up to the Arctic Circle,” explains first author Angélique Lamaze. “There, the flies experience long summer days or even almost constant light, so-called white nights.” Constant lighting disrupts the function of the internal clock because it triggers the permanent degradation of the clock protein TIMELESS via a molecular reaction chain. Thus, the circadian rhythm is lost.
However, temperature cycles can overcome the effects of constant illumination. Our experts have demonstrated that flies with the recently evolved ls-tim allele synchronise their circadian rhythm with the ambient temperature under light and temperature conditions that mimic a Scandinavian summer day. One indicator was the insects’ level of active movement. With constant lighting and a daily temperature cycle between 16 and 25 degrees Celsius, the flies with the ls-tim allele were particularly active in the second half of the warm phase. In contrast, when there was constant lighting and temperature, there was no behavioural rhythm. Unlike the ls-tim flies, those fruit flies carrying only the original gene variant (s-tim) showed no behavioural adaptation — neither with temperature cycling nor a constant temperature.
New gene variant increases reproductive success
“From an evolutionary biology point of view, the behavioural adaptation can be well explained,” emphasizes Angélique Lamaze. “Summer is the insects’ reproductive season. Animals that are synchronised in their behaviour and ready to mate at the same time of day have a better chance of meeting and reproducing. As ls-tim allows such synchronization even under extremely long summer days, this is most likely an important factor contributing to its ongoing northward spread and could explain its evolutionary success. “
The research team also proved that even a single copy of the ls-tim allele is sufficient to enable synchronisation in “Scandinavian conditions,” which is further supporting the spreading of this allele. Like humans, for example, fruit flies possess two, either identical or similar, copies of each gene — one copy from each parent.
The new gene variant produces a protein that deviates slightly from the original form, which is particularly stable under illumination and, therefore, interferes with the molecular reaction cascade that otherwise triggers the degradation of the clock protein TIMELESS. This, the team concluded, is a prerequisite required for the temperature cycle to restore the circadian rhythm of fruit flies under continuous illumination.
The neurobiologists used a combination of behavioural experiments as well as modern genetic and immunohistochemical methods in their study. Among other things, they compared fly strains with different genetic make-ups investigating their behaviour during simulated Scandinavian summer nights and the activity of their clock genes in different brain neurons.