- Manuja Sharma, Lauren K. Lee, Matthew D. Carson, David S. Park, Se W. An, Micah G. Bovenkamp, Jess J. Cayetano, Ian A Berude, Zheng Xu, Alireza Sadr, Shwetak N. Patel, Eric J. Seibel. O-pH: Optical pH Monitor to Measure Oral Biofilm Acidity and Assist in Enamel Health Monitoring. IEEE Transactions on Biomedical Engineering, 2022; 1 DOI: 10.1109/TBME.2022.3153659
The O-pH system is a prototype optical device that emits an LED light and measures the reactions of that light, the fluorescence, with an FDA-approved chemical dye applied to teeth. The O-pH then produces a numerical reading of the pH, or acidity, of the plaque covering those teeth. Knowing how acidic the plaque is can tell dentists and patients what area of a tooth is most at risk of developing a cavity.
“Plaque has a lot of bacteria that produce acid when they interact with the sugar in our food,” said Manuja Sharma, lead author and a doctoral student in the UW Department of Electrical and Computer Engineering. “This acid is what causes the corrosion of the tooth surface and eventually cavities. So, if we can capture information about the acidic activity, we can get an idea of how bacteria are growing in the dental biofilm, or plaque.”
Sharma explained that not all bacteria in that biofilm are bad or will lead to cavities, so measuring the acidity of the environment can tell a dentist what they need to know about the threat of developing problems. That can limit the need to test for specific harmful bacteria, of which there can be a multitude.
To test their device, the researchers recruited 30 patients between the ages of 10 and 18, with a median age of 15, in the UW School of Dentistry’s Center for Pediatric Dentistry. The researchers chose kids for their study in large part because the enamel on kids’ teeth is much thinner than that of adults, so getting early warning of acid erosion is even more important. To perform the measurements with the O-pH device, the researchers also recruited second- and third-year students in the dentistry school, who were supervised by a faculty member.
The test is non-invasive. While the dye is applied to the teeth, at the end of a length of cord is the probe that transmits and collects light while hovering over the surface of a tooth (see photos). The collected light travels back to a central box that provides a pH reading. The conditions on the patients’ teeth were read several times before and after sugar rinses and other condition changes, such as pre- and post-professional dental cleaning.
Eric Seibel, senior author and research professor of mechanical engineering in the UW College of Engineering, said the idea for adding the acidity test as a new clinical procedure came from envisioning that when a patient first sits in the dental chair, before their teeth get cleaned, “a dentist would rinse them with the tasteless fluorescent dye solution and then get their teeth optically scanned to look for high acid production areas where the enamel is getting demineralized.”
The study was published in February in IEEE Transactions on Biomedical Engineering. The research team reported that one limitation to their study was being unable to consistently measure the same location on each tooth during each phase of testing. To address this limitation, in particular, the researchers are evolving their device to a version that produces images for dentists that instantly show the exact location of high acidity, where the next cavity may occur.
“We do need more results to show how effective it is for diagnosis, but it can definitely help us understand some of your oral health quantitatively,” said Sharma. “It can also help educate patients about the effects of sugar on the chemistry of plaque. We can show them, live, what happens, and that is an experience they’ll remember and say, OK, fine, I need to cut down on sugar!”
Co-authors include Lauren Lee, UW Department of Microbiology; Matthew Carson, UW Human Photonics Laboratory; David Park, Se An, Micah Bovenkamp, Jess Cayetano, Ian Berude, Zheng Xu, Alireza Sadr, UW School of Dentistry; and Shwetak Patel, UW Electrical and Computer Engineering, Paul. G. Allen School of Computer Science. This research was funded by the National Science Foundation, Oral Health Monitor, Institute of Translational Health Sciences; and the National Center for Advancing Translational Sciences of the National Institutes of Health.